近日,北京市報告兩例猴痘病毒感染病例,其中一例為境外輸入病例,另一例為境外輸入病例的關(guān)聯(lián)病例,兩名病例是通過親密接觸感染。那么猴痘到底是什么?猴痘病毒又有哪些特征?針對猴痘的臨床研究都有哪些呢?
猴痘是由猴痘病毒引起的人獸共患病。猴痘病毒和天花一樣,屬于正痘病毒屬,1958年首次在用于研究猴子群落中發(fā)現(xiàn),因此命名。目前,猴痘病毒已經(jīng)在人與人之間傳播。
猴痘病毒( Monkeypox,Mpox)具有正痘病毒的典型形態(tài),形態(tài)與正痘病毒一致, 外形為圓角磚形或卵圓形,大小為200~400nm。磚形或卵圓狀,外被脂蛋白膜或封套,中間具有兩個含蛋白質(zhì)的側(cè)體,有1個厚膜核心,核心內(nèi)含很大的雙鏈DNA基因體。猴痘病毒的基因組為雙鏈DNA,長約197kb,基因組末端包含一個相同但方向相反的末端反向重復(fù)序列。該病毒包含190個開放閱讀框,其中4個位于末端反向重復(fù)序列中。
猴痘病毒核心蛋白:A29L, A35R, B6R, M1R, H3L
其實(shí)痘病毒存在兩種形式:成熟病毒粒子(mature virion,MV) 和脂質(zhì)膜 (extracellular enveloped,EV),EV有一層來自內(nèi)質(zhì)網(wǎng)膜的包膜,此包膜在與細(xì)胞接觸后分解,露出外膜進(jìn)而直接和細(xì)胞膜發(fā)生融合,隨后進(jìn)行下游的感染。而MV可以直接和細(xì)胞膜進(jìn)行融合,至少有12種MV參與融合,同時可以通過巨胞飲內(nèi)吞進(jìn)入細(xì)胞,外膜與內(nèi)吞體膜在內(nèi)吞體里融合釋放出內(nèi)膜包繞的病毒核心。復(fù)制的場所是帶孔洞的內(nèi)膜中進(jìn)行,之后孔洞閉合,病毒粒子進(jìn)行下一步的成熟形成典型的痘病毒啞鈴形態(tài),標(biāo)志MV正式形成。最終完成的病毒粒子可以有兩種方式離開細(xì)胞:最主要的一種為細(xì)胞裂解,MV直接釋放。另一種是利用細(xì)胞的分泌轉(zhuǎn)運(yùn)系統(tǒng),通過高爾基體分泌至細(xì)胞外,這種方式會使得病毒額外獲得一層被膜產(chǎn)生EV。
痘病毒復(fù)制周期
普健生物聯(lián)合法國Proteogenix頂級科學(xué)團(tuán)隊(duì),雙方通過對猴痘病毒( Monkeypox,Mpox)和痘苗病毒(vaccinia virus,VACV)的基因序列進(jìn)行深入研究成功設(shè)計開發(fā)真核系統(tǒng)表達(dá)的猴痘病毒( Monkeypox,Mpox)系列核心蛋白A29L、A35R、B6R、M1R、E8L以及相關(guān)具有生物學(xué)活性的中和抗體產(chǎn)品,助力疫苗、藥物、診斷試劑等不同方向的研究。
真核表達(dá)猴痘核心蛋白及相關(guān)中和抗體
Monkeypox virus/MPXV A29L
A29L是細(xì)胞內(nèi)成熟病毒(IMV)表面包膜蛋白(與牛痘病毒VACV,A27同源)。溶瘤痘苗病毒(VV)屬于痘病毒科中一擁有192kb雙螺旋DNA遺傳物質(zhì)的病毒,會在細(xì)胞質(zhì)中繁殖出具有鞘膜的病毒,進(jìn)而感染其他不同種的哺乳動物細(xì)胞及個體.VV中的A27L基因能表達(dá)出一14kDa大小的鞘膜蛋白,此蛋白能與細(xì)胞表面上的硫酸乙酰肝素(Heparan Sulfate)作用使病毒跟細(xì)胞結(jié)合并且可催化細(xì)胞融合。
Monkeypox virus/MPXV A29L
Catalog No:
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Name:Recombinant Monkeypox virus/MPXV A29L Protein, C-His
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Predicted molecular weight:15.33 kDa
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Expression system: Mammalian
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Protein length: Asp2-Glu110
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Protein length: Asp2-Glu110
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SDS PAGE Purity:90%
SDS PAGE for Recombinant Monkeypox virus/MPXV A29L Protein
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Anti- Monkeypox virus/MPXV A29L Antibody
Catalog No:
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Name:Anti-Monkeypox virus/MPXV A29L Antibody (SAA0286)
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Host species:Mouse
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Applications:ELISA,Neut
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Species reactivity:MPXV
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Stock:In stock
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Detects Monkeypox virus/MPXV A29L in direct ELISAs
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Monkeypox virus/MPXV A35R
A35R是細(xì)胞內(nèi)包膜病毒粒子(IEV)和細(xì)胞外包膜病毒粒子(EEV)的包膜成分,與脊索痘病毒A33R同源。痘苗病毒中的A33R在病毒粒子在細(xì)胞間的傳播中起著至關(guān)重要的作用,是疫苗開發(fā)的重要靶點(diǎn),也有研究表明A35R是猴痘病毒(Monkeypox virus,MPXV)血清學(xué)檢測的潛在靶點(diǎn)。
Recombinant Monkeypox virus/MPXV A35R Protein
Catalog No:
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Name:Recombinant Monkeypox virus/MPXV A35R Protein, C-His
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Predicted molecular weight:16.65 kDa
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Expression system: Mammalian
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Protein length: Val57-Thr181
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Stock:In stock
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SDS PAGE Purity:90%
SDS PAGE for Recombinant Monkeypox virus/MPXV A35R Protein
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Anti-Monkeypox virus/MPXV A35R Antibody (SAA0287)
Catalog No:
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Name:Anti-Monkeypox virus/MPXV A35R Antibody (SAA0287)
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Host species:Mouse
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Applications:ELISA,Neut
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Species reactivity:MPXV
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Stock:In stock
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Detects Monkeypox virus/MPXV A35R in direct ELISAs.
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Monkeypox virus/MPXV B6R
B6R是宿主范圍蛋白,位于細(xì)胞外包膜病毒粒子(EEV)的膜上,與牛痘病毒補(bǔ)體控制蛋白B5同源,參與補(bǔ)體激活的負(fù)調(diào)節(jié)。研究表明B5依賴于與A33或A34的相互作用,以定位到細(xì)胞內(nèi)包膜的位點(diǎn),并能相互結(jié)合釋放到細(xì)胞外病毒體的包膜中。B5對于有效生產(chǎn)感染性EV至關(guān)重要,A33,A34,B5三者復(fù)合物在ER退出以及三種糖蛋白正確定位到包裹的細(xì)胞內(nèi)部位中起作用。
Recombinant Monkeypox virus/MPXV B6R/SL-159 Protein
Catalog No:
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Name:Recombinant Monkeypox virus/MPXV B6R/SL-159 Protein, C-His
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Predicted molecular weight:31.73 kDa
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Expression system: Mammalian
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Protein length: Thr20-His279
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Stock:In stock
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SDS PAGE Purity:90%
SDS PAGE for Recombinant Monkeypox virus/MPXV B6R/SL-159 Protein
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Monkeypox virus/MPXV M1R
M1R與牛痘病毒L1蛋白同源,是一種在成熟IMV顆粒表面發(fā)現(xiàn)的跨膜蛋白。由L1R ORF編碼,高度保守,參與病毒粒子組裝,在病毒進(jìn)入和成熟過程中發(fā)揮重要作用。L1是誘導(dǎo)低pH觸發(fā)的細(xì)胞-細(xì)胞融合所必需的,且對牛痘病毒復(fù)制至關(guān)重要。
Recombinant Monkeypox virus/MPXV M1R Protein
Catalog No:
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Name:Recombinant Monkeypox virus/MPXV M1R Protein, C-His
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Predicted molecular weight:22.35 kDa
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Expression system: Mammalian
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Protein length: Gly2-Gly183
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Stock:In stock
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SDS PAGE Purity:90%
SDS PAGE for Recombinant Monkeypox virus/MPXV M1R Protein
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Anti-Monkeypox virus/MPXV M1R Antibody
Catalog No:
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Name:Anti-Monkeypox virus/MPXV M1R Antibody (SAA0283)
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Host species:Mouse
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Applications:ELISA,Neut
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Species reactivity:MPXV
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Stock:In stock
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Detects Monkeypox virus/MPXV M1R in direct ELISAs.
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Monkeypox virus/MPXV E8L
E8L是病毒入侵宿主細(xì)胞重要基因,在病毒入侵宿主完成復(fù)制的過程中作為MV的細(xì)胞表面結(jié)合蛋白與細(xì)胞表面的硫酸軟骨素結(jié)合,以提供病毒粒子附著在靶細(xì)胞上。有研究表明利用siRNA技術(shù)能有效拮抗Monkeypox virus的A6R和E8L基因,由此構(gòu)建siA6-a和si8 -d能破壞MPV復(fù)制。
Recombinant Monkeypox virus/MPXV E8L Protein
Catalog No:
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Name:Recombinant Monkeypox virus/MPXV E8L Protein, C-His
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Predicted molecular weight:22.35 kDa
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Expression system: Mammalian
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Protein length: Gly2-Gly183
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Stock:In stock
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SDS PAGE Purity:90%
SDS PAGE for Recombinant Monkeypox virus/MPXV E8L Protein
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Anti-Monkeypox virus/MPXV E8L Antibody
Catalog No:
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Name:Anti-Monkeypox virus/MPXV E8L Antibody (SAA0285)
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Host species:Mouse
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Applications:ELISA,Neut
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Species reactivity:MPXV
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Stock:In stock
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Detects Monkeypox virus/MPXV E8L in direct ELISAs.
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Monkeypox virus/MPXV H3L
H3L是一種324個AA長的糖基轉(zhuǎn)移酶,位于病毒包膜上,在與宿主細(xì)胞的附著和隨后的痘病毒進(jìn)入中起關(guān)鍵作用。研究表明,天花疫苗誘導(dǎo)的抗體與H3蛋白結(jié)合,分化簇 (CD8)+ T細(xì)胞將H3L識別為抗原,表明其免疫學(xué)意義。
Recombinant Monkeypox virus/MPXV H3L Protein
Catalog No:
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Name:Recombinant Monkeypox virus/MPXV H3L Protein, C-His
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Predicted molecular weight:35.46 kDa
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Expression system: Mammalian
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Protein length: Ala2-Phe282
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Stock:In stock
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SDS PAGE Purity:90%
SDS PAGE for Recombinant Monkeypox virus/MPXV H3L Protein
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普健生物聯(lián)合法國Proteogenix頂級科學(xué)團(tuán)隊(duì),雙方通過對猴痘病毒( Monkeypox virus(MPXV))和痘苗病毒的基因序列進(jìn)行深入研究成功設(shè)計開發(fā)不同系統(tǒng)表達(dá)的猴痘病毒( Monkeypox virus(MPXV))系列核心蛋白A29L、A35R、B6R、M1R、H3L、E8L以及相關(guān)抗體和檢測試劑盒產(chǎn)品助力疫苗、藥物、診斷試劑等不同方向的研究。
我們還有在研發(fā)配對猴痘病毒(Monkeypox virus/MPXV )相關(guān)試劑盒系列;
猴痘病毒(Monkeypox virus/MPXV )抗原檢測試劑盒:
Cat. #
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Product name
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KVV13401
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Monkeypox Virus (MPXV) A29L Antigen ELISA Kit
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KVV13301
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Monkeypox Virus (MPXV) M1R Antigen ELISA Kit
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KVV12701
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Monkeypox Virus (MPXV) H3L Antigen ELISA Kit
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KVV13201
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Monkeypox Virus (MPXV) E8L Antigen ELISA Kit
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KVV13101
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Monkeypox Virus (MPXV) A35R Antigen ELISA Kit
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猴痘病毒(Monkeypox virus/MPXV )抗體檢測試劑盒
Cat. #
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Product name
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KAV13401
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Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (A29L)
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KAV13301
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Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit(M1R)
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KAV12701
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Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit(H3L)
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KAV13201
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Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit(E8L)
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KAV13101
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Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit(A35R)
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參考文獻(xiàn):
[1]Subunit recombinant vaccine protects against monkeypox. J Immunol. 2006 Aug 15;177(4):2552-64. doi: 10.4049/jimmunol.177.4.2552. PMID: 16888017.
[2]Outbreak of human monkeypox in Nigeria in 2017-18: a clinical and epidemiological report. Lancet Infect Dis. 2019 Aug;19(8):872-879. doi: 10.1016/S1473-3099(19)30294-4. Epub 2019 Jul 5. PMID: 31285143.
[3]A Nucleic Acid-Based Orthopoxvirus Vaccine Targeting the Vaccinia Virus L1, A27, B5, and A33 Proteins Protects Rabbits against Lethal Rabbitpox Virus Aerosol Challenge. J Virol. 2022 Feb 9;96(3):e0150421. doi: 10.1128/JVI.01504-21. Epub 2021 Dec 1. PMID: 34851148; PMCID: PMC8826804.
[4]Mapping and functional analysis of interaction sites within the cytoplasmic domains of the vaccinia virus A33R and A36R envelope proteins. J Virol. 2003 Apr;77(7):4113-26. doi: 10.1128/jvi.77.7.4113-4126.2003. PMID: 12634370; PMCID: PMC150634.
[5]Vaccinia virus induces cell fusion at acid pH and this activity is mediated by the N-terminus of the 14-kDa virus envelope protein. Virology. 1990 Sep;178(1):81-91. doi: 10.1016/0042-6822(90)90381-z. PMID: 2389560.
[6]Molecular Evidence of Human Monkeypox Virus Infection, Sierra Leone. Emerg Infect Dis. 2019 Jun;25(6):1220-1222. doi: 10.3201/eid2506.180296. Epub 2019 Jun 17. PMID: 30900976; PMCID: PMC6537715.
[7]Inhibition of monkeypox virus replication by RNA interference. Virol J. 2009 Nov 4;6:188. doi: 10.1186/1743-422X-6-188. PMID: 19889227; PMCID: PMC2777875.
[8]Rapid protection in a monkeypox model by a single injection of a replication-deficient vaccinia virus. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10889-94. doi: 10.1073/pnas.0804985105. Epub 2008 Aug 4. PMID: 18678911; PMCID: PMC2495015.