重組蛋白表達(dá)系列一

哺乳動物細(xì)胞表達(dá)系統(tǒng)

展示案例：HEK293F Expression System

 Project Information 

（1）Protein Name: protein L（一種scfv）

該蛋白不易表達(dá)，采用哺乳動物細(xì)胞表達(dá)系統(tǒng)表達(dá)活性最佳，其他表達(dá)系統(tǒng)表達(dá)活性低。

（2）Expression vector: pATX2

（3）Expression host: HEK293F

總結(jié)：蛋白L（scfv）采用普健高表達(dá)pATX系列載體配合公司優(yōu)化高密度哺乳表達(dá)系統(tǒng)Xten293，成功表達(dá)，表達(dá)量高達(dá)31.2mg/L，純度>90%，經(jīng)檢驗，其具有較高活性。

以圖為鑒

Test Report

01

Expression test

MW. Molecular weight marker. ?. Non-transfected (negative control).

The protein L expressed secretorily in HEK293F cells.

02

Purification Test

MW. Molecular weight marker. IN. Input.  FT. Flow through. W. Washes. E. Eluted fractions.

A. The purification test result. B. Final QC gel.

L-pATX2 was transfected with 80ml HEK293F cells. The culture medium and cells which were collected on the sixth day post transfection did purification test.

03

Fermentation and Purification

1L scale up purification

MW. Molecular weight marker. IN. Input.  FT. Flow through. W. Washes. E. Eluted fractions.

A. The purification test result. B. Final QC gel.